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Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae): is the insect canonical sequence conserved?

by: de Castro, Carini Picardi Moraes; Cardoso, Danon Clemes; Micolino, Ricardo; Cristiano, Maykon Passos

Publication date: 2020-8-14
Usage: Attribution 4.0 International
Topics: evolution, FISH, insects, leafcutter ants, telomere
Publisher: Pensoft Publishers
Collection: biodiversity
Contributor: Pensoft Publishers
Language: English
Rights: https://biodiversitylibrary.org/permissions
Rights-holder: Copyright held by individual article author(s).
Volume: 14
Item Size: 17.1M

Abstract: Telomeric sequences are conserved across species. The most common sequence reported among insects is (TTAGG)n, but its universal occurrence is not a consensus because other canonical motifs have been reported. In the present study, we used fluorescence in situ hybridization (FISH) using telomeric probes with (TTAGG)6 repeats to describe the telomere composition of leafcutter ants. We performed the molecular cytogenetic characterization of six Acromyrmex Mayr, 1865 and one Atta Fabricius, 1804 species (Acromyrmex ambiguus (Emery, 1888), Ac. crassispinus (Forel, 1909), Ac. lundii (Guérin-Mèneville, 1838), Ac. nigrosetosus (Forel, 1908), Ac. rugosus (Smith, 1858), Ac. subterraneus subterraneus (Forel, 1893), and Atta sexdens (Linnaeus, 1758)) and described it using a karyomorphometric approach on their chromosomes. The diploid chromosome number 2n = 38 was found in all Acromyrmex species, and the karyotypic formulas were as follows: Ac. ambiguus 2K = 14M + 12SM + 8ST + 4A, Ac. crassispinus 2K = 12M + 20SM + 4ST + 2A, Ac. lundii 2K = 10M + 14SM + 10ST + 4A, Ac. nigrosetosus 2K = 12M + 14SM + 10ST + 2A, and Ac. subterraneus subterraneus 2K = 14M + 18SM + 4ST + 2A. The exact karyotypic formula was not established for Ac. rugosus. FISH analyses revealed the telomeric regions in all the chromosomes of the species studied in the present work were marked by the (TTAGG)6 sequence. These results reinforce the premise that Formicidae presents high homology between their genera for the presence of the canonical sequence (TTAGG)n.
Addeddate: 2025-03-16 11:09:58
Bhl_virtual_titleid: 210879
Bhl_virtual_volume: v.14:no.3 (2020)
Call number: 10_3897_CompCytogen_v14i3_52726
Call-number: 10_3897_CompCytogen_v14i3_52726
Foldoutcount: 0
Genre: article
Identifier: comparativefish14deca
Identifier-ark: ark:/13960/s2mrd8jhrc0
Identifier-bib: 10_3897_CompCytogen_v14i3_52726
Identifier-doi: 10.3897/CompCytogen.v14i3.52726
Issue: 3
Ocr: tesseract 5.3.0-6-g76ae
Ocr_detected_lang: en
Ocr_detected_lang_conf: 1.0000
Ocr_detected_script: Latin
Ocr_detected_script_conf: 1.0000
Ocr_module_version: 0.0.21
Ocr_parameters: -l eng
Page_number_confidence: 70
Page_number_module_version: 1.0.5
Page_range: 369-385
Pages: 17
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Pdf_module_version: 0.0.25
Possible copyright status: In copyright. Digitized with the permission of the rights holder.
Ppi: 300
Source: Comparative Cytogenetics 14(3)
Year: 2020